49 US AI Startups Raise $100 Million or More in 2025

Hrvoje Milakovic
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Venture capital pours into artificial intelligence as U.S. startups secure massive funding rounds amid fierce competition for talent and compute resources. Forty-nine companies cross the $100 million threshold this year, matching 2024’s count but with heightened activity in mega-deals exceeding $1 billion. These investments signal sustained momentum in AI infrastructure, healthcare applications, and enterprise tools.

The tally encompasses diverse sectors, from AI research labs to specialized hardware firms. Total funding surpasses $25 billion across series A through F rounds, with valuations reaching $183 billion for frontrunners. Announcements peak in the third quarter, reflecting accelerated deployment of large language models and agentic systems.

Anysphere, developer of the Cursor coding platform, clinches a $2.3 billion series C on November 13, valuing the company at $29.3 billion. Parallel raises $100 million in series A funding on November 12, co-led by Index Ventures and Kleiner Perkins, to build web infrastructure for AI agents. Hippocratic AI follows with $126 million series C on November 3, led by Avenir Growth, targeting healthcare agents at a $3.5 billion valuation.

Fireworks AI secures $250 million series C on October 28, reaching $4 billion valuation for its open-source model platform. Uniphore’s $260 million series F on October 22 includes investments from Snowflake Ventures and Nvidia, bolstering enterprise AI at $2.5 billion. Sesame nets $250 million series B on October 21, co-led by Sequoia and Spark Capital, for voice AI advancements.

OpenEvidence, a medical chatbot firm in Cambridge, Massachusetts, raises $200 million series C on October 20, hitting $6 billion valuation. Lila Sciences garners $350 million series A on October 14, co-led by Braidwell and Collective Global, for science superintelligence platforms. Reflection AI, a DeepSeek rival, commands $2 billion series B on October 9, led by Nvidia, at $8 billion.

EvenUp obtains $150 million series E on October 7, led by Bessemer, for personal injury legal AI exceeding $2 billion valuation. Periodic Labs launches with $300 million seed on September 30, led by Felicis and Andreessen Horowitz, to develop AI scientists. Cerebras Systems raises $1.1 billion series G on September 30, co-led by Fidelity, for AI infrastructure at $8.1 billion.

Modular collects $250 million on September 24, led by US Innovative Technology Fund with GV participation. Distyl AI raises $175 million series B on September 23, valued at $1.8 billion, including Khosla Ventures. Upscale AI starts with $100 million seed on September 17, co-led by Maverick Silicon and Mayfield.

Groq achieves $750 million series E on September 17, led by Disruptive, nearing $6.9 billion for AI inference. Invisible Technologies secures $100 million on September 16, valued at $2 billion, led by Vanara Capital. Cognition AI raises $400 million series C on September 8, led by Founders Fund, for vibe-coding agent Devin at $10.2 billion.

Baseten obtains $150 million series D on September 5, led by Bond, at $2.1 billion for AI infrastructure. Sierra raises $350 million on September 4, led by Greenoaks Capital, surpassing $10 billion for customer service agents. You.com gains $100 million series C on September 3, led by Cox Enterprises, at $1.5 billion for personalized search.

Anthropic commands $13 billion series F on September 2, led by Iconiq and Fidelity, at $183 billion. EliseAI raises $250 million series E on August 20, led by Andreessen Horowitz, at $2.2 billion for healthcare automation. Decart secures $100 million on August 7, valued at $3.1 billion, with Sequoia participation.

Fal raises $125 million series C on July 31, led by Meritech, at $1.5 billion for generative media. Ambience Healthcare collects $243 million series C, led by Oak HC/FT, for AI operating systems. Reka AI nets $110 million series B on July 22, reaching $1 billion with Snowflake and Nvidia.

Thinking Machines Lab launches with $2 billion seed on July 15, led by Andreessen Horowitz, at $12 billion. OpenEvidence adds $210 million series B on July 15, led by Kleiner Perkins, at $3.5 billion. Harmonic raises $100 million series B on July 10, led by Kleiner Perkins, at $875 million for mathematical reasoning.

Abridge secures $300 million series E on June 24, led by Andreessen Horowitz, at $5.3 billion for healthcare transcription. Harvey raises $300 million series E on June 23, co-led by Kleiner Perkins, at $5 billion for legal tools. Tennr obtains $101 million series C, led by IVP, at $605 million.

Glean raises $150 million series F on June 10, led by Wellington Management, at $7.25 billion for enterprise search. Anysphere adds $900 million series C on June 5, led by Thrive Capital, nearing $10 billion. Snorkel AI secures $100 million series D on May 29, led by Addition, at $1.3 billion.

LMArena raises $100 million seed on May 21, co-led by Andreessen Horowitz, at $600 million for AI benchmarking. TensorWave collects $100 million series A on May 14, co-led by Magnetar and AMD Ventures. SandboxAQ raises $450 million series E on April 4, including Nvidia, at $5.7 billion.

Runway secures $308 million series D on April 3, led by General Atlantic, at $3 billion for media AI. OpenAI raises $40 billion on March 31, led by SoftBank, at $300 billion. Nexthop AI nets $110 million series A on March 25, led by Lightspeed.

Insilico Medicine raises $110 million series E on March 13, co-led by Value Partners, at $1 billion for drug discovery. Celestial AI collects $250 million series C on March 11, led by Fidelity, at $2.5 billion. Lila Sciences adds $200 million seed, led by Flagship Pioneering.

Reflection.Ai raises $130 million series A on March unspecified, led by Lightspeed, at $580 million for autonomous systems. Turing secures $111 million series E on March 7, led by Khazanah Nasional, at $2.2 billion. Shield AI raises $240 million series F on March 6, co-led by L3Harris, at $5.3 billion.

Anthropic adds $3.5 billion series E on March 3, led by Lightspeed, at $61.5 billion. Together AI raises $305 million series B on February 20, co-led by Prosperity7, at $3.3 billion. Lambda secures $480 million series D on February 19, co-led by SGW, nearing $2.5 billion.

Abridge adds $250 million series D on February 17, co-led by IVP, at $2.75 billion. Eudia raises $105 million series A on February 13, led by General Catalyst. EnCharge AI nets $100 million series B on February 13, led by Tiger Global.

Harvey adds $300 million series D on February 12, led by Sequoia, at $3 billion. ElevenLabs raises $180 million series C on January 30, co-led by ICONIQ, over $3 billion. Hippocratic AI starts with $141 million series B on January 9, led by Kleiner Perkins, over $1.6 billion.

These rounds underscore investor appetite for scalable AI technologies, with repeat fundings indicating rapid growth trajectories.

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Tristan has a strong interest in the intersection of artificial intelligence and creative expression. He has a background in computer science, and he enjoys exploring the ways in which AI can enhance and augment human creativity. In his writing, he often delves into the ways in which AI is being used to generate original works of fiction and poetry, as well as to analyze and understand patterns in existing texts.

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